Gene Knockout Mutations in E.coli Using Bacteriophage P1vir

In a skibidi lab, the goal was to create gene knockouts in E.coli using P1vir phage. These goon viruses are nature's champs, infecting bacteria like no other, lowkey taking over the microbial world. The procedure kicked off with some E.coli cultures, mixing in that dank bacteriophage buffer. The action unfolded with nagC and rpoA phages, each targeting their specific E.coli. After a chill in the 28°C water bath, a sigma-level incubation at 37°C followed, flipping those tubes like a pro. Finally, the plates were spread and left to chill overnight, waiting for those colonies to pop. A true edge in microbiology, this experiment unlocked the potential of phages in genetic engineering.